Journal:

Article Title: Atypical Genetic Locus Associated with Constitutive Production of Enterocin B by Enterococcus faecium BFE 900

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: The immunity of L. sake DSM 20017 containing either pCMAP04 or pCMAP05 (Table ) was confirmed with the transformants as indicators in deferred inhibition assays with E. faecalis ATCC 19433 containing pCMAP03 as the producer strain.

Techniques: Plasmid Preparation, BAC Assay, Expressing

Journal:

Article Title: Atypical Genetic Locus Associated with Constitutive Production of Enterocin B by Enterococcus faecium BFE 900

doi:

Figure Lengend Snippet: Deferred-inhibition test with E. faecalis ATCC 19433 containing pCMAP03 (EntB+) against L. sake DSM 20017 containing plasmids pMG36e (A), pCMAP04 (B), and pCMAP05 (C).

Article Snippet: The immunity of L. sake DSM 20017 containing either pCMAP04 or pCMAP05 (Table ) was confirmed with the transformants as indicators in deferred inhibition assays with E. faecalis ATCC 19433 containing pCMAP03 as the producer strain.

Techniques: Inhibition

Journal: International Journal of Molecular Sciences

Article Title: Modulation of ESKAPE Bacteria Properties by NK-92 and NK-92-Derived LEVs: First Insights

doi: 10.3390/ijms27093953

Figure Lengend Snippet: Effect of NK-92 cells on E. faecium colony formation and growth. Colony formation assay: E. faecium plated alone (Control) or co-cultured with NK-92 cells (Treated). ( Left ) Raw plate images; ( Right ) automated analysis (Scan500, Interscience, Saint Nom la Bretèche, France). T 0 /T 1 : plating time points, E + 0n = 10 n .

Article Snippet: Also, we used ESKAPE group bacteria: Enterococcus faecium (19434), Staphylococcus aureus (29213), Klebsiella pneumoniae (13883), Acinetobacter baumannii (19606), Pseudomonas aeruginosa (27853) and Enterobacter spp. (13047) (ATCC, USA) and cultured them on agarose medium, under appropriate biosafety containment, in accordance with institutional safety protocols for handling pathogenic microorganisms.

Techniques: Colony Assay, Control, Cell Culture

Journal: Scientific Reports

Article Title: Evaluation of mdh , dld , tcfA , and folE gene markers for detection of enteric fever using real-time PCR

doi: 10.1038/s41598-026-35011-9

Figure Lengend Snippet: Real-time PCR amplification and confirmation of the 760-bp mdh gene in S. enterica isolates. ( A ) Amplification curves demonstrating consistent SYBR Green fluorescence and exponential amplification across many test samples. ( B ) Ct values and concentration estimations for the detection of the mdh gene in qPCR assays across all isolates. The range of Ct value is 16.68 to 22.56. ( C ) A 760 bp anticipated amplification result is exhibited by 1.5% agarose gel electrophoresis for all samples (lanes 1–22); M denotes the 1k bp DNA ladder; Lane 1 to 15: Salmonella species; Lane 16: Klebsiella pneumoniae ; Lane 17: Escherichia coli ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. Lane 21: S. Typhi ATCC 700,931; Lane 22 non-template control (NTC).

Article Snippet: Lane 16: Klebsiella pneumoniae ; Lane 17: Escherichia coli ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. The amplification in the strain of S. Typhi ATCC 700,931 is confirmed by lane 21.

Techniques: Real-time Polymerase Chain Reaction, Amplification, SYBR Green Assay, Fluorescence, Concentration Assay, Agarose Gel Electrophoresis, Control

Journal: Scientific Reports

Article Title: Evaluation of mdh , dld , tcfA , and folE gene markers for detection of enteric fever using real-time PCR

doi: 10.1038/s41598-026-35011-9

Figure Lengend Snippet: ( A ) SYBR Green chemical amplification graphs exhibiting positive reactions with fluorescence surpassing the threshold. ( B ) Ct value for each sample in the assay range from 14.51 to 28.27. ( C ) Gel electrophoresis confirms the amplification of a 992 bp fragment in several Salmonella isolates; M1 functions as a 1 kb marker; Lane 1 to 15: Salmonella species; Lane 16: Escherichia coli ; Lane 17: Klebsiella pneumoniae ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. Lane 21: S. Typhi ATCC 700,931; Lane 22 non-template control (NTC).

Article Snippet: Lane 16: Klebsiella pneumoniae ; Lane 17: Escherichia coli ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. The amplification in the strain of S. Typhi ATCC 700,931 is confirmed by lane 21.

Techniques: SYBR Green Assay, Amplification, Fluorescence, Nucleic Acid Electrophoresis, Marker, Control

Journal: Scientific Reports

Article Title: Evaluation of mdh , dld , tcfA , and folE gene markers for detection of enteric fever using real-time PCR

doi: 10.1038/s41598-026-35011-9

Figure Lengend Snippet: Real-time PCR detection of Salmonella Typhi using the tcfA gene. ( A ) Amplification curves demonstrate a clear rise in fluorescence signals in positive samples, while negative controls exhibit no amplification. ( B ) Ct values from separate reactions ranged from 13.97 to 31.25, demonstrating effective detection and analysis of Salmonella species DNA across diverse samples. ( C ) Agarose gel (1.5%) examination of qPCR results verifies the amplification of the approximately 663 bp tcfA motif in positive samples (lanes 1–15); Lanes 16–20 indicate negative results for non- Salmonella species. Lane 16: Klebsiella pneumoniae ; Lane 17: Escherichia coli ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. Lane 21 confirms the amplification in the S. Typhi ATCC 700,931 strain. Lane 22 shows a negative result in the non-template control (NTC); M1 and M2 signify 100 bp DNA marker, while M3 indicates 1 kb DNA marker.

Article Snippet: Lane 16: Klebsiella pneumoniae ; Lane 17: Escherichia coli ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. The amplification in the strain of S. Typhi ATCC 700,931 is confirmed by lane 21.

Techniques: Real-time Polymerase Chain Reaction, Amplification, Fluorescence, Agarose Gel Electrophoresis, Control, Marker

Journal: Scientific Reports

Article Title: Evaluation of mdh , dld , tcfA , and folE gene markers for detection of enteric fever using real-time PCR

doi: 10.1038/s41598-026-35011-9

Figure Lengend Snippet: ( A ) Amplification curves show that positive samples clearly show an increase in fluorescence signals, whilst negative controls show no amplification. ( B ) The effective identification and analysis of Salmonella species DNA across a variety of samples was demonstrated by the Ct values from distinct reactions, which ranged from 13.50 to 30.39 ( C ) Analysis of qPCR findings on an Agarose gel (1.5%) confirms that the about 276 bp motif in Salmonella species (lanes 1–15) was amplified. Results for non-Salmonella species are shown negatively in lanes 16–20. Lane 16: Klebsiella pneumoniae ; Lane 17: Escherichia coli ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. The amplification in the strain of S. Typhi ATCC 700,931 is confirmed by lane 21. In non-template control (NTC), lane 22 displays a negative result; M1 denotes a 1 kb DNA marker.

Article Snippet: Lane 16: Klebsiella pneumoniae ; Lane 17: Escherichia coli ; Lane 18: Staphylococcus aureus ; Lane 19: Acinetobacter sp. Lane 20: Enterococcus sp. The amplification in the strain of S. Typhi ATCC 700,931 is confirmed by lane 21.

Techniques: Amplification, Fluorescence, Agarose Gel Electrophoresis, Control, Marker